DNA
Part:BBa_K2100027:Design
Designed by: Colleen Foley Group: iGEM16_MIT (2016-10-16)
pEXPR pERE3:eYFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190
Illegal PstI site found at 428 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190
Illegal PstI site found at 428 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190
Illegal PstI site found at 428 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190
Illegal PstI site found at 428
Illegal AgeI site found at 211 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
design comments.
Source
This is synthesized by us.
References
[1] Sathya G, Li W, Klinge CM, Anolik JH, Hilf R, Bambara RA. Effects of multiple estrogen responsive elements, their spacing, and location on estrogen response of reporter genes. Mol Endocrinol. 1997;11(13):1994–2003.