DNA

Part:BBa_K2100027:Design

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-16)


pEXPR pERE3:eYFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal PstI site found at 428
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal PstI site found at 428
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal PstI site found at 428
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal PstI site found at 428
    Illegal AgeI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

design comments.


Source

This is synthesized by us.

References

[1] Sathya G, Li W, Klinge CM, Anolik JH, Hilf R, Bambara RA. Effects of multiple estrogen responsive elements, their spacing, and location on estrogen response of reporter genes. Mol Endocrinol. 1997;11(13):1994–2003.